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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Fig. 5

Effect of hAECs-secreting cytokines on TGF-β/SMAD pathway of human luteinized granulosa cells in vitro. a ELISA assay was measured TGF-β 1 concentration in conditioned medium derived from GCs, which co-cultured with hAECs for 24 hours. bf SMAD protein expressions in human GCs were detected by Western blot after co-cultured with hAECs for 24 hours. hAEC-secreting cytokines activated the expression of P-SMAD2 and P-SMAD3 in granulosa cells. Data represent means ± SEM. Control group (n = 4); CTX group (n = 4); CTX + hAECs group (n = 4). * P < 0.05; ** P < 0.01; *** P < 0.001

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