Table 1 Primary and secondary antibodies used for MPC ± PPS cytometric analysis
Primary antibodies | Type | Origin |
|---|---|---|
Stro-1 | In-house antibody | Provided by Prof. S. Gronthos and Prof. A. Zannettino |
CD73 | Purified mouse anti-human CD73 | BD Pharmingen 550256 |
CD90 | Biotin mouse anti-human CD90 | BD Pharmingen 555594 |
CD105 | Purified mouse anti-human CD105 | BD Pharmingen 555690 |
CD44 (H9H11) | In-house antibody | Provided by Prof. S. Gronthos and Prof A. Zannettino |
CD146 (CC9) | In-house antibody | |
CD34 | CD34 FITC | Beckman Coulter IM1870 |
CD45 | CD45 FITC | Beckman Coulter IM0782U |
CD14 | CD14 FITC | Beckman Coulter IM0645U |
Secondary antibodies | ||
 Streptavidin FITC conjugate |  | Invitrogen SA1001 |
 IgM FITC | Goat anti-mouse IgM FITC | Southern Biotech 1020-02 |
 IgG FITC | Goat anti-mouse IgG FITC | Southern Biotech 1030-02 |
Negative Controls | ||
 IgM | 1A6.12 isotype-matched negative control/anti-salmonella | Provided by Dr. L Ashman |
 IgG1 | 1B5 isotype-matched negative control/anti-salmonella | Provided by Dr. L Ashman |
 IgG2a | 1D4.5 isotype-matched negative control/anti-salmonella | Provided by Dr. L Ashman |