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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

Fig. 6

Evaluation of the cross-reaction between LXR and Hh signaling. Cells were transfected with either LXRα or LXRβ-specific siRNAs or a negative control siRNA for 24 h and further incubated with SS (1 μM) for 48 h, and protein levels of (a) LXRα and (b) LXRβ were assessed. c Cells were transfected with both LXRα and LXRβ-specific siRNAs to a final concentration of 100 nM of each siRNA prior to SS treatment, and protein levels of Smo and Gli1 were analyzed. d Protein levels of LXRα and LXRβ measured after cells were pretreated with 22-NHC before SS treatment. Representative result from five independent experiments. LXR liver X receptor, NC negative control, 22-NHC 22-azacholesterol, siRNA small interfering RNA, SS 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol

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