Fig. 2

Immunohistochemical staining showing hPDLSCs in the subretinal space. Cross-sections of the eyes removed 2 weeks (a–c) and 8 weeks (h–m) post transplantation were stained for TRA-1-85. Section without primary antibody served as negative control staining (h–j). Cell nuclei counterstained with DAPI (a, h, k), and merged images (c, j, m). d–g hPDLSCs coexpressed PKCα (e) and TRA-1-85 (f). Cell nuclei counterstained with DAPI (d), and merged images (g). a–g Scale bar = 50 μm. h–m Scale bar = 20 μm. Inserts show higher magnification of the boxed area. Scale bars in insets = 20 μm. ONL outer nuclear layer, INL inner nuclear layer, GCL ganglion cell layer