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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: TGF-β1-induced chondrogenesis of bone marrow mesenchymal stem cells is promoted by low-intensity pulsed ultrasound through the integrin-mTOR signaling pathway

Fig. 3

Identification of TGF-β1-induced chondrogenesis. a Immunofluorescence staining of COL2 and toluidine blue staining in BMSCs cultured with basic or chondrogenic medium (basic medium treated with different doses of TGF-β1) on day 10; scale bars = 100 μm or 25 μm. b qRT-PCR analysis of COL2, aggrecan, SOX9, and COL1. The values are mean ± SEM of triplicate experiments. n = 4; *P < 0.05. COL1 collagen type-I, COL2 collagen type-2. GAPDH glyceraldehyde 3-phosphate dehydrogenase, LIPUS low-intensity pulsed ultrasound, SOX9 SRY-related high mobility group-box gene 9, TGF transforming growth factor

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Stem Cell Research & Therapy

ISSN: 1757-6512