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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Mesenchymal stem cells decrease lung inflammation during sepsis, acting through inhibition of the MAPK pathway

Fig. 6

Co-cultivation with MSCs inhibited LPS-induced phosphorylation of RSK, p38, ERK, and NF-κB expression in macrophages. Macrophages were treated with LPS (1 μg/mL) for 15, 30, and 60 min. The MAPK phosphorylation and NF-κB expression were evaluated by Western blot analysis. a RSK was phosphorylated at 15, 30, and 60 min in response to LPS. The co-culture with mesenchymal stem cells (MSCs) inhibited the activation-associated phosphorylation of RSK at all times. b p38 phosphorylation was increased in the macrophages treated with LPS after 15, 30, and 60 min, and decreased upon co-cultivation with MSCs at 15 and 30 min. c ERK was phosphorylated after 15 and 30 min in response to LPS stimulation and this phosphorylation could be inhibited by co-cultivation with MSCs. Blots are representative of at least four separate experiments. d NF-κB expression was increased in the LPS group after 15, 30, and 60 min. This increase was inhibited by MSCs at all times. Data represent the mean ± SEM, n = 5. ## p < 0.01, ### p < 0.001, versus corresponding untreated controls; *p < 0.05, **p < 0.01, versus corresponding RAW counterparts

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