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Table 1 Cell lines and maximum passages for feeder-free hPSC culture and subsequent RPE and LESC differentiations

From: Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method

Cell line (hESC/hiPSC)

Maximum FF passage

Cell line (hESC/hiPSC)

FF passages for RPE differentiation

Regea08/017 (hESC1)

28

(31 (p25: 46;XX 20q tel*))

Regea08/017 (hESC1)

5, 8, 10, 22, 24

Regea14/010 (hESC2)

11

UTA.10902.EURCCs (hiPSC1)

5, 7

UTA.10902.EURCCs (hiPSC1)

10

  

UTA.04607.WT (hiPSC2)

15

  

UTA.04511.WT (hiPSC3)

29 (p23: 46;XY 20q11**)

Cell line (hESC/hiPSC)

FF passages for LESC differentiation

Hel24.3 (hiPSC4)

19

Regea08/017 (hESC1)

6, 6, 8

UTA.10802.EURCCs (hiPSC5)

4

UTA.04607.WT (hiPSC2)

3, 6, 13

UTA.04311.WT (hiPSC6)

11

  
  1. *Changes at the q telomere of chromosome 20
  2. **Translocation at 20q11
  3. FF feeder-free, hESC human embryonic stem cell, hiPSC human induced pluripotent stem cell, LESC limbal epithelial stem cell, p passage, RPE retinal pigment epithelium