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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats

Fig. 3

In vivo hAD-MSC tracking. a PKH26-labeled hAD-MSCs showed red fluorescence (100×). b,c The labeling rates of PKH26-labeled hAD-MSCs (b) and their subcultured cells (c) were detected by flow cytometry. d The growth curves of PKH26-labeled and unlabeled hAD-MSCs were measured by CCK-8 assay (n = 6). e–g Transplanted hAD-MSCs were observed at 24 h (e), 4 weeks (f), and 8 weeks (g) after cell transplantation in ovaries (100×). Scale bars = 100 μm. *P < 0.05, **P < 0.01. hAD-MSCs human adipose-derived mesenchymal stem cells, LIPUS low-intensity pulsed ultrasound, POI primary ovarian insufficiency

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