Fig. 2
From: Automated image analysis detects aging in clinical-grade mesenchymal stromal cell cultures
Growth kinetics from long-term continuous cultures of MSCs. Mesenchymal stromal cells (MSCs) were isolated from bone marrow aspirates collected from healthy volunteer donors (n = 6; MSC-1 to MSC-6) and cultured continuously from primary cultures (passage 0) to senescence. a Cumulative population doubling numbers (PD) are presented as a function of culturing time. Each point in the growth curve indicates an individual cell passaging event starting from passage 0. b The rate of cell division is presented as PD/culturing day per passage (n = 6). c Representative morphology and culture characteristics of long-term MSC cultures from passages 1, 3, 5, 7, and 9 (donor MSC-6) imaged using 4× magnification. The respective passage (p) numbers are shown in each micrograph