Fig. 5
From: Automated image analysis detects aging in clinical-grade mesenchymal stromal cell cultures
The expression of aging-related markers is increased at the onset of senescence. a A representative image of p16INK4a and p21Cip1/Waf1 Western blot analyses (n = 6). Equal amounts of total protein (20 μg) were loaded onto the gel, and β-actin was used as a loading control. b Senescence-associated β-galactosidase (SA-β-gal) activity was measured from three donors using a quantitative activity assay. Equal amounts of total protein were used per sample, and all samples were analyzed in triplicate. The results are presented as relative fluorometric units (RFU). Bonferroni-corrected Student’s t test, ***p < 0.001. Mean telomere length was measured from six donors using terminal restriction fragment analysis (TRF) and presented as in function of c population doubling (PD) numbers and d passage. Mean telomere length is shown as kilobase pairs (kbp)