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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Differentiation of nonhuman primate pluripotent stem cells into functional keratinocytes

Fig. 1

Differentiation and characterization of monkey keratinocytes derived from PSCs. a Schematic of protocol design for differentiation of monkey and human pluripotent stem cells into keratinocytes. Black triangles represent medium change (containing RA and BMP4) and white triangles depict end of differentiation. b Monkey and c human K-PSC phase-contrast microscopy analysis (scale bar: 100 μm) and immunofluorescence analysis for keratinocyte markers: keratin 5 (K5) and keratin 14 (K14) (scale bar: 50 μm). Adult keratinocytes represented as control. d Representative fluorescence-activated cell sorting analysis. Keratinocyte suspensions were fixed, permeabilized and stained with K5 and a panel of cytokeratin 14, 15, 16 and 19 (PAN-keratin) in keratinocytes derived from pluripotent stem cells and adult keratinocytes as biological control. Isotype-specific antibodies used as control. Cells analyzed on a MACSQuant Analyzers system (10,000 events per experiment). e Quantitative PCR analysis of NANOG, ΔNp63, KRT5, KRT14, INTA6 and KRT10 in monkey and human K-PSCs and adult keratinocytes. DAPI 4′,6-diamidino-2-phenylindole, ESC embryonic stem cells, H human, iPSC induced pluripotent stem cells, K-ESC keratinocytes derived from embryonic stem cells, K-iPSC keratinocytes derived from induced pluripotent stem cells, M monkey

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