Fig. 3
From: Differentiation of nonhuman primate pluripotent stem cells into functional keratinocytes
Expression of MHC-I antigens in human and monkey PSC-derived keratinocytes and epidermis. a Representative fluorescence-activated cell sorting analysis of major histocompatibility complex class I (MHC-I) in monkey and human keratinocytes derived from ESCs and iPSCs and in adult keratinocytes. b Immunofluorescence analysis of the expression of MHC-I antigens in in-vitro reconstructed monkey and human epidermis from K-ESCs, K-iPSCs (at passage 1) and adult keratinocytes (scale bar: 20 μm). c TaqMan gene expression assay by qPCR analysis of monkey specific MHC-I genes: Mamu A and Mamu B in monkey keratinocytes derived from pluripotent stem cells and in-vitro reconstructed epidermis (at passage 1). Results expressed as relative expression to monkey PSCs. DAPI 4′,6-diamidino-2-phenylindole, ESC embryonic stem cell, iPSC induced pluripotent stem cell, K-ESC keratinocytes derived from embryonic stem cells, K-iPSC keratinocytes derived from induced pluripotent stem cells