From: Immunoprivileged no more: measuring the immunogenicity of allogeneic adult mesenchymal stem cells
Assay | Effector cell | Target cell | Immune function measured | Outcome measurement |
---|---|---|---|---|
Ex-vivo MLR | Lymphocytes (primarily CD4+) | Splenocytes/lymphocytes | Cell-mediated | T-cell proliferation |
Cytotoxicity | Cytotoxic T lymphocytes | MSCs | Cell-mediated | Target cell death |
ELISPOT | T or B cell | Splenocytes/lymphocytes | Cell-mediated or humoral | Cytokine secretion |
Antibody-dependent CDC | B cell | Lymphocytes or MSCs | Humoral | Target cell death |
In vivo imaging | Â | MSCs | Â | MSC survival |