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Table 2 Assays for measuring cell-mediated and humoral immune responses against allogeneic MSCs

From: Immunoprivileged no more: measuring the immunogenicity of allogeneic adult mesenchymal stem cells

Assay

Effector cell

Target cell

Immune function measured

Outcome measurement

Ex-vivo MLR

Lymphocytes (primarily CD4+)

Splenocytes/lymphocytes

Cell-mediated

T-cell proliferation

Cytotoxicity

Cytotoxic T lymphocytes

MSCs

Cell-mediated

Target cell death

ELISPOT

T or B cell

Splenocytes/lymphocytes

Cell-mediated or humoral

Cytokine secretion

Antibody-dependent CDC

B cell

Lymphocytes or MSCs

Humoral

Target cell death

In vivo imaging

 

MSCs

 

MSC survival

  1. CDC complement-dependent cytotoxicity, ELISPOT enzyme-linked immunospot, MLR mixed leukocyte reaction, MSC mesenchymal stem cell