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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human liver organoids generated with single donor-derived multiple cells rescue mice from acute liver failure

Fig. 4

Single donor cell-derived LOs improve survival in mice with ALF. a Schematic representation of the protocol for generating ALF mice and transplanting SDC-LOs. b ALT and AST were detected on days 0 and 2 in the sham (n = 3) and SDC-LO-transplanted (n = 4) ALF mice. c Kaplan–Meier survival curves of ALF mice in the sham (n = 10) and SDC-LO-transplanted (n = 10) groups. d Macroscopic images (upper panel; scale bars, 5 mm) and hematoxylin and eosin (HE) staining (lower panel; scale bars, 50 μm) of the liver in normal mice, ALF mice, sham ALF mice, and SDC-LO-transplanted ALF mice on day 2. Red arrowhead, hepatocellular necrosis; black arrowhead, hepatocellular steatosis; blue arrowhead, enlarged hepatocyte. e Human-specific ALB was analyzed on days 2 and 7 in the sham (n = 3) and SDC-LO-transplanted (n = 8) groups. f HE staining of transplants on day 14. Scale bars, 50 μm. g Immunofluorescent analysis of human ALB and A1AT expression in transplants on day 14. Scale bars, 50 μm. *p < 0.05, ***p < 0.001. hALB human albumin, ALF acute liver failure, DT diphtheria toxin, ns not significant. N.D. not determined, SDC-LO single donor cell-derived liver organoid, Tx treatment, ALT alanine aminotransferase, AST aspartate aminotransferase

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