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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: ERK inhibition promotes neuroectodermal precursor commitment by blocking self-renewal and primitive streak formation of the epiblast

Fig. 2

PD0325901 in 2i/LIF was responsible for neural differentiation of EpiSCs. a Activities of signal pathways were detected by western blot analysis. β-catenin and STAT3 were activated by 3 μM CHIR99021 and 1000 U/ml LIF, respectively; Phospho-ERK1/2 was inhibited by 1 μM PD0325901. b Real-time PCR results showed that PD0325901 in 2i/LIF induced neuroectoderm marker Sox1, and inhibited pluripotent marker Oct4 and primitive streak markers T and Mixl1. c PD0325901 treatment for 24 hours dramatically increased the proportion of GFP-positive cells, when Sox1-GFP EpiSCs were cultured in N2B27 medium. GFP-positive cells were detected by fluorescence microscopy. Bar, 100 μm. d, e Sox1-GFP EpiSCs were cultured in N2B27 containing 0.01% DMSO or 1 μM PD0325901 for 24 hours. GFP-positive cells were analyzed by FACS. **p < 0.01. DMSO dimethyl sulfoxide, EpiSC epiblast stem cell, ERK extracellular signal-regulated protein kinase, GFP green fluorescent protein, DIC differential interference contrast, LIF leukemia inhibitory factor, Mixl1 mix paired-like homeobox, Oct4 octamer-binding transcription factor 4, T brachyury, Sox1 sex determining region Y-box 1, STAT3 signal transducer and activator of transcription 3

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