Fig. 3

Effect of EFS on viability of liver cells. Effect of different concentrations of exosome-rich fractionated secretome (EFS) on viability of HepG2 cells over a period of 72 h (a). MTT assay to check effect of fractionated secretome and unfractionated secretome on viability of injury-induced liver cells in 2D tissue culture plate (2D-TCP) (b) and 3D cryogel scaffold (3D-pNC CRYOGEL) (c). LDH activity of acetaminophen (APAP) and hydrogen peroxide (HP) injury-induced liver cells before and after treatment with unfractionated MSC secretome (MSC-CM) and fractionated MSC secretome (EFS) (d). Statistical analysis: n = 3, **p < 0.01. 2D two dimensional, 3D three dimensional, MSC mesenchymal stromal cell, CM conditioned medium, LDH lactate dehydrogenase