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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stromal cell-derived exosome-rich fractionated secretome confers a hepatoprotective effect in liver injury

Fig. 5

Effect of EFS on oxidative stress induced during liver injury. Quantitative DCFDA assay for reactive oxygen species (ROS) released by liver cells post treatment with acetaminophen (APAP) and hydrogen peroxide (HP) in the presence of unfractionated MSC secretome (MSC-CM) and fractionated MSC secretome (EFS) in 2D tissue culture plate (2D-TCP) (a) and 3D cryogel scaffold (3D-pNC CRYOGEL) (b). Qualitative DCFDA assay for ROS 24 h post treatment with 8 mM APAP (c), 8 mM APAP in presence of unfractionated MSC secretome (d), 8 mM APAP in presence of fractionated MSC secretome (e) and without any treatment (f). Qualitative DCFDA assay for ROS 24 h post treatment with 60 μM hydrogen peroxide (H2O2) (g), 60 μM H2O2 in presence of unfractionated MSC secretome (h), 60 μM H2O2 in presence of fractionated MSC secretome (i) and without any treatment (j). Scale bar for all microscopic images: 100 μm. Statistical analysis: n = 3, **p < 0.01, ***p < 0.001. MSC mesenchymal stromal cell, CM conditioned medium, EFS exosome-rich fractionated secretome, 2D two dimensional, 3D three dimensional

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