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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Changes in the secretome of tri-dimensional spheroid-cultured human mesenchymal stem cells in vitro by interleukin-1 priming

Fig. 1

Formation and measurements of spheroids. a Scheme of the formation of spheroids, with representative images at different times of growth. b Haematoxylin and eosin staining of a 20-μm section of a spheroid, in a bright field image. c Immunostaining of the same section using DAPI, indicating a compact cellular structure. d Spheroids were measured at different time points by measuring the maximum sectional area, indicated in the image as a dashed line. e Areas of main spheroids (mean + SEM) were larger at early time points and decreased with time, indicating the compaction of the spheroid. Scale bars = 500 μm in a, 200 μm in b–d. ****p < 0.001. P passage

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