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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Targeted reversion of induced pluripotent stem cells from patients with human cleidocranial dysplasia improves bone regeneration in a rat calvarial bone defect model

Fig. 2

Osteoblastic differentiation of induced pluripotent stem cells (iPSCs) in vitro. a Schedule of osteogenic differentiation. b Runt-related transcription factor 2 (RUNX2) localization by immunofluorescent microscopy. Single cells from embryoid bodies (EBs) were cultured with osteoblast differentiation medium (OBM) containing cytokines for 12 days and stained for RUNX2 (green) and the nuclei (blue). c Staining of alkaline phosphatase (ALP) activity (days (d)0, 3, 6, 9, and 12). d qRT-PCR analysis of RUNX2 target genes. e qRT-PCR analysis of RUNX2 and transcription factors. Values are presented as the mean ± SD (n = 3). *p < 0.05. FGF fibroblast growth factor, HOB human osteoblast, IGF insulin-like growth factor, TGF transforming growth factor

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