Table 1 Primers used for quantitative reverse transcriptase polymerase chain reaction
Gene symbol | GenBank accession no. | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|
RUNX2 | NM_001024630.2 | gtgcctaggcgcatttca | gctcttcttactgagagtggaagg |
ALP | NM_000478.3 | caaccctggggaggagac | gcattggtgttgtacgtcttg |
COL1A1 | NM_000088.3 | gggattccctggacctaaag | gggattccctggacctaaag |
OSX | NM_152860.1 | catctgcctggctccttg | caggggactggagccata |
OC | NM_199173.3 | tgagagccctcacactcctc | acctttgctggactctgcac |
MSX2 | NM_002449.4 | tcggaaaattcagaagatgga | caggtggtagggctcatatgtc |
DLX5 | NM_005221.5 | ctacaaccgcgtcccaag | gccattcaccattctcacct |
DLX3 | NM_005220.2 | gagcctcctaccggcaatac | tcctccttcaccgacactg |
TWIST1 | NM_000474.3 | agctacgccttctcggtct | ccttctctggaaacaatgacatc |
18SrRNA | M11188.1 | cggacaggattgacagattg | cgctccaccaactaagaacg |