Table 1 Antibodies used for flow cytometry, immunofluorescence, and western blot analysis
Flow cytometry | Source | Immunofluorescence | Source |
|---|---|---|---|
CD44-PeCy7 | 560569, BD Pharmingen | SM22a | ab10135, Abcam |
CD73 | 550256, BD Pharmingen | Calpomin | Ab700, Abcam |
CD105 | 555690, BD Pharmingen | SM-MHC | M7786, Sigma |
HLA-ABC-APC | 562006, BD Pharmingen | α-SMA | A2547, Sigma |
CD34-PerCP-Cy5.5 | 347222, BD Pharmingen | Tert (1:50) | AP33476PU-N, Acris |
CD45-APC | 555485, BD Pharmingen | Sox2 | PA1-16968, Thermo |
CD49a | 559594, BD Pharmingen | Nanog | PA1-097X, Thermo |
CD146-PE | 561013, BD Pharmingen | Oct3/4 | sc-5279, Santa Cruz Biotechnology |
CD166-PE | 559263, BD Pharmingen | Western blot assay | Source |
CD19 | 555410, BD Pharmingen | ||
CD56-PeCy7 | 557747, BD Pharmingen | Tert(1:1000) | SAB4502945, Sigma |
CD80-PE | 560925, BD Pharmingen | Sox2 (1:2000) | PA1-16968, Thermo |
CD83-PeCy7 | 561132, BD Pharmingen | Oct3/4(1:1000) | sc-5273, Santa Cruz Biotechnology |
CD86-V450 | 560359, BD Pharmingen | Nanog (1:2000) | PA1-097X, Thermo |
Strol | 14-6688-80, eBiosciences | Erk1/2 (1:1000) | 9102, Cell Signaling |
CD106-PerCPCy5.5 | 45-0149-42, eBiosciences | pErk1/2(1:1000) | 9101, Cell Signaling |
CD14-PerCPCy5.5 | 45-0149-42, eBiosciences | β-Catenin (1:5000) | ab32572, Abcam |
CD40-PeCy7 | 25-0409-41, eBiosciences | Smad2/3 (1:400) | AF3797, R&D Systems |
CD275(B7H2)-PE | 12-5889-41, eBiosciences | pSmad2/3(1:1000) | 8828, Cell Signaling |
CD90-APC | 17-0909-42, eBiosciences | NICD(1:500) | ab8925, Abcam |
HLA-DR-efluor450 | 48-9956-41, eBiosciences | Akt1/2(1:1000) | sc-1619, Santa Cruz Biotechnology |
α-SMA | A2547, Sigma | pArk1/2(1:1000) | 9271, Cell Signaling |
SM22α | ab10135, Abcam | Smad1/5/9(1:2000) | ab66737, Abcam |
vWF | ab8822, Abcam | PSmad1/5(1:1000) | 9516, Cell Signaling |