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Table 2 Primers used for real-time quantitative PCR

From: Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta

 

Forward sequence (5′–3′)

Reverse sequence (5′–3′)

GAPDH

GAAGGTGAAGGTCGGAGTCA

AATGAAGGGGTCATTGATGG

SOX2

GAGAGTGTTTGCAAAAGGGG

TGGGGCTCAAACTTCTTCTC

SOX2 (2)

CCACCTACAGCATGTCCTACTCG

GGGAGGAAGAGGTAACCACAGG

OCT4

GCAGAAGAGGATCACCCTGG

AAAGCGGCAGATGGTCGTTT

OCT4 (2)

CTGCACAGATATGCAAAGCAG

TGATCTGCTGCAGTGTGGGT

NANOG

CTTGCCTTGCTTTGAAGCAT

TTCTTGACCGGGACCTTGTC

NANOG (2)

ACCTCAGCTACAAACAGGT

AAAGGCTGGGGTAGGTAGGT

hTERT

CGGAAGAGTGTCTGGAGCAA

GGATGAAGCGGAGTCTGGA

hTERT splicing variant

GCC TGA GCT GTA CTT TGT CAA

CGC AAA CAG CTT GTTCTC CAT GTC

ERK1/2 (MAPK1)

GCTAGATTCCAGCCAGGATACA

AGAACACCGATGTCTGAGCA

AKT1

ATGAGCGACGTGGCTATTGT

CCTCACGTTGGTCCACATCC

CSHL1

CTGTGGACAGCTCACCTAGC

AGCCTGGATAAGGGAACGGT

SMAD2

GTGGCAGGCGGGTCTAC

GCAAGCCACGCTAGGAAAAC

SMAD8

GTGGCCAACCTGTAGATGCC

CTCCCCAACTCGGTTGTTCA

β-Catenin

GGAGGAAGGTCTGAGGAGCAG

ATTGTCCACGCTGGATTTTCAA

SMAD1/5/9

CAGAGTGGCCAACCTGTAGA

TCCCCAACTCGGTTGTTCAG

  1. GAPDH glyceraldehyde 3-phosphate dehydrogenase, SOX2 sex determining region Y-box 2, OCT4 octamer-binding transcription factor 4, hTERT human telomerase reverse transcriptase, ERK extracellular signal-regulated kinase 2, also known as mitogen-activated protein kinase 1 (MAPK1), AKT protein kinase B, CSHL chorionic somatomammotropin hormone like 1, SMAD, mothers against decapentaplegic homolog
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Stem Cell Research & Therapy

ISSN: 1757-6512