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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells

Fig. 1

Design of CRISPR/dCas9-E nucleases specific for targeting the ectodysplasin (EDA) promotor. a Schematic representation of the EDA genome and b scheme of lentiCRISPR/dCas9-E plasmids. c Schematic representation of the pLKO.1-puro-U6 vector. A guide-sequence insertion site existed downstream of the U6 promoter for cloning the designed single-guide RNA (sgRNA) by the BfuAI restriction site

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