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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells

Fig. 6

The effect of dCas9-E BM-MSCs on the regeneration of sweat glands in vivo. a The saline-treated paw (Sham) showed a negative result for the perspiration test, whereas paws implanted with Dox-induced dCas9-E BM-MSCs showed a positive result (sgRNA2). b Hematoxylin and eosin staining was used to detect the duct structure of sweat gland tissue after injection with Dox-induced dCas9-E BM-MSCs. c The expression of carcinoembryonic antigen (CEA; red), cytokeratin 19 (CK19; green) and nucleus (DAPI) in BM-MSCs after implantation for 20 days. d The quantification of each paw (0.5 cm × 0.5 cm, treated with collagenase I for 1 h at 37 °C) was also conducted after treatment with Dox-induced dCas9-E BM-MSCs. **p < 0.01 for Dox-induced dCas9-E BM-MSCs group versus normal group. Scale bar = 50 μm. sgRNA single-guide RNA

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