Fig. 1

a Schematic overview of the protocols used for the differentiation of hPSCs into pancreatic progenitors. hPSCs are differentiated through the stages of definitive endoderm (DE) and posterior foregut to yield pancreatic progenitors through two different protocols differing in their duration of stage 3 treatment. Each of these protocols were either dissociated after generation of DE or left unmanipulated throughout the differentiation stages. Pancreatic progenitors generated using protocol 2 (P2) following dissociation were further differentiated through stage 5 to generate endocrine progenitors in adherent culture. b Representative immunofluorescent images of hESC-derived DE expressing high levels of SOX17 (green) and FOXA2 (red) after stage 1 of differentiation. The differentiated cells lost their pluripotency as indicated by the loss of OCT4 at the end of stage 1 (c). Nuclei are labeled with Hoechst. d Flow cytometry analysis of SOX17 expression at the end of stage 1 of differentiation. All data shown are representative results from at least three independent experiments. Scale bars = 100 μm