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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1

Fig. 2

Generation of PDX1+/NKX6.1+ pancreatic progenitors using different protocols. Immunostaining of PDX1 (green) and NKX6.1 (red) in hESC-derived pancreatic progenitors generated from protocol 1 non-dissociated (P1-ND) (a), protocol 2 non-dissociated (P2-ND) (b), protocol 1 dissociated (P1-D) (c), and protocol 2 dissociated (P2-D) (d). Note the high co-expression level of PDX1 and NKX6.1 in the P2-D in comparison to other protocols. Nuclei are labeled with Hoechst. e Flow cytometry analysis for the expression of PDX1 and NKX6.1 after stage 4 of differentiation. f Flow cytometry analysis for the expression of SOX9 after stage 4 of differentiation. Scale bars = 100 μm

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