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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1

Fig. 5

Enhancement of pancreatic progenitor differentiation is associated with an increase in cell proliferation. Flow cytometry analysis of BrdU incorporation (6 h) and DNA content in dissociated and non-dissociated cells at day 5 (a) and day 10 (b) of differentiation into pancreatic progenitors. Flow cytometry analysis for the co-expression of Ki67 (proliferation marker) with NKX6.1 (c) and SOX9 (d) after stage 4 of differentiation in P1-ND and P2-D progenitors. Double immunofluorescence staining for Ki67 and PDX1 (e) in novel pancreatic progenitors generated from P2-D protocol. Scale bars = 50 μm. P1-D protocol 1 dissociated, P1-ND protocol 1 non-dissociated, P2-D protocol 2 dissociated, P2-ND protocol 2 non-dissociated

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