Fig. 5From: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1Enhancement of pancreatic progenitor differentiation is associated with an increase in cell proliferation. Flow cytometry analysis of BrdU incorporation (6 h) and DNA content in dissociated and non-dissociated cells at day 5 (a) and day 10 (b) of differentiation into pancreatic progenitors. Flow cytometry analysis for the co-expression of Ki67 (proliferation marker) with NKX6.1 (c) and SOX9 (d) after stage 4 of differentiation in P1-ND and P2-D progenitors. Double immunofluorescence staining for Ki67 and PDX1 (e) in novel pancreatic progenitors generated from P2-D protocol. Scale bars = 50 μm. P1-D protocol 1 dissociated, P1-ND protocol 1 non-dissociated, P2-D protocol 2 dissociated, P2-ND protocol 2 non-dissociatedBack to article page