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Table 2 Primer sequences for real-time polymerase chain reaction

From: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1

Gene Primer sequence
PDX1 5’-CGTCCAGCTGCCTTTCCCAT-3’
5’-CCGTGAGATGTACTTGTTGAATAGGA-3’
NKX6.1 5’-GGGCTCGTTTGGCCTATTCGTT-3’
5’-CCACTTGGTCCGGCGGTTCT-3’
SOX9 5’-GACTACACCGACCACCAGAACTCC-3’
5’-GTCTGCGGGATGGAAGGGA-3’
FOXA2 5’-GGGAGCGGTGAAGATGGA-3’
5’-TCATGTTGCTCACGGAGGAGTA-3’
HNF6 5’-GGACCTCAAGATAGCAGGTTTAT-3’
5’-CAGAATGCAGGTGAGCTAAGT-3’
HNF1β 5’-ACACACCTCCCATCCTCAAG-3’
5’-CATTTTAGCAGCCCTCCAAG-3’
NGN3 5’-GGCTGTGGGTGCTAAGGGTAAG-3’
5’-CAGGGAGAAGCAGAAGGAACAA-3’
NEUROD1 5’-GCCCCAGGGTTATGAGACTAT-3’
5’-GAGAACTGAGACACTCGTCTGT-3’
NKX2.2 5’-GGCCTTCAGTACTCCCTGCA-3’
5’-GGGACTTGGAGCTTGAGTCCT-3’
AFP 5’-ACAGAGGAACAACTTGAGGCTGTC-3’
5’-AGCAAAGCAGACTTCCTGTTCCTG-3’
ALB 5’-GTGAAACACAAGCCCAAGGCAACA-3’
5’-TCAGCCTTGCAGCACTTCTCTACA-3’