Fig. 4From: Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitroEffects of hucMSC-CM on expression of E-cadherin, Collagen-I, and α-SMA in each group 14 days postoperatively. A Immunohistochemical staining of kidney tissue sections using E-cadherin (a–d), Collagen-I (e–h), and α-SMA (i–l). B–D Total IOD for E-cadherin, Collagen-I, and α-SMA in each group. E Western blot analysis of E-cadherin, Collagen-I, and α-SMA protein in each group. F–H Relative protein expression levels in kidney tissues. β-actin used as inner reference and quantified using densitometric analysis. Values presented as mean ± SD. UUO group compared with Sham, Control, and hucMSC-CM groups. Values statistically significant at: *P < 0.05, Sham and Control groups compared with UUO and hucMSC-CM groups; #P < 0.05. CM conditioned medium, hucMSC human umbilical cord-derived mesenchymal stem cell, IOD integrated optical density, SMA smooth muscle actin, UUO unilateral ureteral obstructionBack to article page