Fig. 7From: Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitroEffects of hucMSC-CM on EMT process of NRK-52E cells in each group at 48 hours. a Representative micrographs from each group. b–d Immunofluorescent staining for cytokeratin 8, E-cadherin, and α-SMA in each group. e Western blot analysis of cytokeratin 8, E-cadherin, and α-SMA protein in each group. f–h Relative protein expression levels in NRK-52E cells. β-actin used as inner reference and quantified using densitometric analysis. Values presented as mean ± SD. TGF-β1 group compared with Control and hucMSC-CM groups. Values statistically significant at: *P < 0.05, Control group compared with TGF-β1 and hucMSC-CM groups; #P < 0.05. CM conditioned medium, DAPI 4′,6-diamidino-2-phenylindole, hucMSC human umbilical cord-derived mesenchymal stem cell, SMA smooth muscle actin, TGF-β1 transforming growth factor β1, UUO unilateral ureteral obstructionBack to article page