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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions

Fig. 4

In-vivo subcutaneous transplantation of ex-vivo-expanded DPSCs cultured in xenogeneic serum-free or FBS-containing culture medium. HA scaffolds containing DPSCs cultured in XFM, SCM, or HA alone (control) were evaluated histologically by HE, MT, and immunohistochemical staining 16 weeks after transplantation. Arrows indicate cells embedded within the newly formed hard tissue, which is outlined by dashed lines. Primary antibody was omitted during immunostaining (negative control). Scale bars, 50 μm. DSP dentin sialoprotein, SCM xenogeneic serum-containing culture medium, XFM xenogeneic serum-free culture medium, HE hematoxylin and eosin, MT Masson’s trichrome, HA hydroxyapatite/β-tricalciumphosphate

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