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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions

Fig. 5

Cellular behavior of DPSCs at overconfluence under xenogeneic serum-free or FBS-containing culture medium. a TUNEL and immunohistochemical staining of overconfluent DPSC cultures in XFM and SCM. Scale bars, 100 μm. b Flow-cytometric analysis of overconfluent XFM and SCM cultures using an Annexin V/PI system. c Quantification of the apoptotic cells. *P < 0.01. d BrdU staining. e Quantification of BrdU-positive cells in XFM and SCM cultures on day 6 (D6) and day 14 (D14) post seeding. Scale bars, 100 μm. *P < 0.01. f Time-course gene-expression profile for apoptosis and cell cycle regulators during cell-growth evaluation of XFM and SCM cells as determined by RT-PCR. SCM xenogeneic serum-containing culture medium, XFM xenogeneic serum-free culture medium, DAPI 4′,6-diamidino-2-phenylindole, TUNEL terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, Bcl-2 B-cell lymphoma 2, BAX B-cell lymphoma-2 associated X, BrdU bromodeoxyuridine, N.S. no significant difference

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