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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions

Fig. 6

Cellular-behavioral analysis after subculture of overconfluent DPSCs cultured in xenogeneic serum-free or FBS-containing culture medium. Phase-contrast images of subcultured overconfluent monolayered SCM cells (Ph-SCM) and subcultured overconfluent multilayered XFM cells (Ph-XFM). Scale bars, 50 μm. SA β-gal staining of a cell aggregate derived from subcultured overconfluent XFM cells (SA β-gal). Scale bar, 50 μm. Histological evaluation of a cell aggregate by HE, TUNEL, and PAS staining. Arrows indicate condensed nuclei according to HE staining (scale bar, 50 μm) and TUNEL-positive cells (scale bar, 20 μm). Arrowheads indicate PAS-positive lipofuscin granules. Scale bar, 20 μm. SCM xenogeneic serum-containing culture medium, XFM xenogeneic serum-free culture medium, SA β-gal senescence-associated β-galactosidase, HE Hematoxylin and eosin, TUNEL Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, PAS, Periodic acid–Schiff

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