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Table 1 Primer sequences and amplification conditions for RT-PCR analysis

From: Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions

Gene

Primer sequences, 5′–3′

Product size (bp)

Annealing temp. (°C)

PCR cycles

GenBank accession number

Vimentin

S:

GGGACCTCTACGAGGAGGAG

200

55

35

NM_003380

A:

CGCATTGTCAACATCCTGTC

    

Runx2

S:

CCCCACGACAACCGCACCAT

292

55

35

NM_004348

A:

GTCCACTCCGGCCCACAAATC

    

Collagen I

S:

CCAAATCTGTCTCCCCAGAA

214

55

35

NM_000088

A:

TCAAAAACGAAGGGGAGATG

    

Nestin

S:

AACAGCGACGGAGGTCTCTA

220

55

35

NM_006617

A:

TTCTCTTGTCCCGCAGACTT

    

Nanog

S:

ACCTTCCAATGTGGAGCAAC

199

55

35

NM_024865

A:

GAATTTGGCTGGAACTGCAT

    

Oct3/4

S:

GACAGGGGGAGGGGAGGAGCTAGG

144

60

35

NM_001173531

A:

CTTCCCTCCAACCAGTTGCCCCAAAC

    

Sox2

S:

AACCCCAAGATGCACAACTC

152

60

40

NM_003106

A:

CGGGGCCGGTATTTATAATC

    

OCN

S:

GCTGAGTCCTGAGCAGCAG

323

60

30

NM_199173

A:

CGATAGGCCTCCTGAAAGC

    

DSPP

S:

CATTTGGGCAGTAGCATGGG

170

55

35

NM_014208

A:

CACCTTCATGCACCAGGACA

    

PPARγ

S:

TCGGATCCCTCCTCGGAAAT

399

60

35

AB565476

A:

GCAGGCTCCACTTTGATTGC

    

FABP4

S:

TGGGCCAGGAATTTGACGAA

212

60

35

NM_001442

A:

ACGTCCCTTGGCTTATGCTC

    

Bcl-2

S:

GGTGAACTGGGGGAGGATTG

214

60

35

NM_000633

A:

GAAATCAAACAGAGGCCGCA

    

Bax

S:

AAGAAGCTGAGCGAGTGTCTC

338

60

35

NM_001291428

A:

AGTCGCTTCAGTGACTCGG

    

p53

S:

TACCAGGGCAGCTACGGTTT

572

55

25

AB082923

A:

CCTTTCTTGCGGAGATTCTCT

    

p21

S:

TCAGAACCGGCTGGGGATGT

554

60

35

NM_001291549

A:

AGATGTAGAGCGGGCCTTTG

    

p16

S:

CCCAACGCACCGAATAGT

135

65

35

XM_011517676

A:

CACGGGTCGGGTGAGAGT

    

β-actin

S:

GGACTTCGAGCAAGAGATGG

234

60

30

NM_001101

A:

AGCACTGTGTTGGCGTACAG

    
  1. RT-PCR reverse transcription polymerase chain reaction, S sense, A antisense, Runx2 runt-related transcription factor 2, Oct3/4 POU class 5 homeobox 1 (POU5F1), SOX2 sex determining region Y-box 2, OCN osteocalcin, DSPP dentin sialophosphoprotein, PPARγ peroxisome proliferator-activated receptor gamma, FABP4 fatty acid binding protein 4, Bcl-2 B-cell lymphoma 2, BAX B-cell lymphoma-2 associated X