Gene | Primer sequences, 5′–3′ | Product size (bp) | Annealing temp. (°C) | PCR cycles | GenBank accession number | |
---|---|---|---|---|---|---|
Vimentin | S: | GGGACCTCTACGAGGAGGAG | 200 | 55 | 35 | NM_003380 |
A: | CGCATTGTCAACATCCTGTC | Â | Â | Â | Â | |
Runx2 | S: | CCCCACGACAACCGCACCAT | 292 | 55 | 35 | NM_004348 |
A: | GTCCACTCCGGCCCACAAATC | Â | Â | Â | Â | |
Collagen I | S: | CCAAATCTGTCTCCCCAGAA | 214 | 55 | 35 | NM_000088 |
A: | TCAAAAACGAAGGGGAGATG | Â | Â | Â | Â | |
Nestin | S: | AACAGCGACGGAGGTCTCTA | 220 | 55 | 35 | NM_006617 |
A: | TTCTCTTGTCCCGCAGACTT | Â | Â | Â | Â | |
Nanog | S: | ACCTTCCAATGTGGAGCAAC | 199 | 55 | 35 | NM_024865 |
A: | GAATTTGGCTGGAACTGCAT | Â | Â | Â | Â | |
Oct3/4 | S: | GACAGGGGGAGGGGAGGAGCTAGG | 144 | 60 | 35 | NM_001173531 |
A: | CTTCCCTCCAACCAGTTGCCCCAAAC | Â | Â | Â | Â | |
Sox2 | S: | AACCCCAAGATGCACAACTC | 152 | 60 | 40 | NM_003106 |
A: | CGGGGCCGGTATTTATAATC | Â | Â | Â | Â | |
OCN | S: | GCTGAGTCCTGAGCAGCAG | 323 | 60 | 30 | NM_199173 |
A: | CGATAGGCCTCCTGAAAGC | Â | Â | Â | Â | |
DSPP | S: | CATTTGGGCAGTAGCATGGG | 170 | 55 | 35 | NM_014208 |
A: | CACCTTCATGCACCAGGACA | Â | Â | Â | Â | |
PPARγ | S: | TCGGATCCCTCCTCGGAAAT | 399 | 60 | 35 | AB565476 |
A: | GCAGGCTCCACTTTGATTGC | Â | Â | Â | Â | |
FABP4 | S: | TGGGCCAGGAATTTGACGAA | 212 | 60 | 35 | NM_001442 |
A: | ACGTCCCTTGGCTTATGCTC | Â | Â | Â | Â | |
Bcl-2 | S: | GGTGAACTGGGGGAGGATTG | 214 | 60 | 35 | NM_000633 |
A: | GAAATCAAACAGAGGCCGCA | Â | Â | Â | Â | |
Bax | S: | AAGAAGCTGAGCGAGTGTCTC | 338 | 60 | 35 | NM_001291428 |
A: | AGTCGCTTCAGTGACTCGG | Â | Â | Â | Â | |
p53 | S: | TACCAGGGCAGCTACGGTTT | 572 | 55 | 25 | AB082923 |
A: | CCTTTCTTGCGGAGATTCTCT | Â | Â | Â | Â | |
p21 | S: | TCAGAACCGGCTGGGGATGT | 554 | 60 | 35 | NM_001291549 |
A: | AGATGTAGAGCGGGCCTTTG | Â | Â | Â | Â | |
p16 | S: | CCCAACGCACCGAATAGT | 135 | 65 | 35 | XM_011517676 |
A: | CACGGGTCGGGTGAGAGT | Â | Â | Â | Â | |
β-actin | S: | GGACTTCGAGCAAGAGATGG | 234 | 60 | 30 | NM_001101 |
A: | AGCACTGTGTTGGCGTACAG | Â | Â | Â | Â |