Fig. 1

Phenotypic characterization of CSCs and IGF-1-induced CSC migration and proliferation. a Cells were identified by flow cytometry analysis of the following cell surface markers: c-kit (87.42%), Sca-1 (55.97%), CD34 (1.04%), and CD45 (0.95%). b Representative images of migrated CSCs at 48 h (stained with crystal violet) are shown (×100 magnification). c CSCs were stimulated with 100 ng/ml insulin-like growth factor-1 (IGF-1) for 72 h. A CCK-8 assay was used to analyze cell proliferation. The data were obtained from at least three independent experiments and are expressed as the mean ± SD. *P < 0.05, **P < 0.01, IGF-1 versus the control (CON) group