Fig. 1

Evi1-GFP is a faithful reporter for hematopoietic precursors. a Representative data from FACS analysis of green fluorescent protein (GFP)⁺ cells of Evi1-GFP mouse. The GFP⁺ population was further analyzed by staining lineage, Sca1, and c-Kit markers. b Representative data from FACS analysis of bone marrow stem and progenitor populations of Evi1-GFP mouse. The GFP⁺ populations from Lin-Sca1⁺c-Kit⁺ (LSK) and Lin-Sca1⁺c-Kit^– are compared. c (left) The quantitative analysis from FACS analysis of Evi1-GFP mouse bone marrow. The percentage of GFP⁺ cells in LSK, GMP, CMP, CLP, and lineage positive populations is shown. (right) Representative data from FACS analysis of bone marrow stem and progenitor populations of Evi1-GFP mouse. The GFP⁺ populations from Lin-Sca1⁺c-Kit⁺ and Lin-Sca1⁺c-Kit⁺CD150⁺CD48^– are compared. d Colony formation assay (CFC) was performed with sorted GFP⁺ cells from Evi1-GFP mouse. The number of BFU-E + GEMM (erythroid, granulocyte, erythrocyte, monocyte, megakaryocyte), GM (granulocyte, monocyte), and total colony number is shown. e The limiting dilution assay was performed to measure the number of hematopoietic stem cells (HSCs) in the GFP⁺ population of Evi1-GFP mouse; 100, 500, and 2500 GFP⁺ cells from Evi1-GFP mouse were sorted and transplanted into lethally irradiated mice in combination with 3 × 10⁵ recipient-derived bone marrow (BM) cells. The percentage of donor-derived cells in BM was analyzed 16 weeks after reconstitution. Chimerism greater than 1% donor-derived cells was considered positive engraftment. Values show the number of mice with positive engraftment/total mice transplanted in GFP^– and GFP⁺ groups