Fig. 3

Cytokines/growth factors affect the division pattern of hematopoietic precursors. a–c Representative microscopic data of symmetric renewal, symmetric differentiation, and asymmetric division of green fluorescent protein (GFP)⁺ hematopoietic precursors harvested from Evi1-GFP mouse. The GFP pixel intensity unit (PIU) and the ratio was calculated and is shown. d The summary of division patterns of Evi1-GFP⁺ precursors from three independent experiments. The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP⁺ precursors under base conditions is shown. Error bars represent standard error of the mean (SEM). e Representative microscopic data are shown for GFP⁺ cells treated with STIF combinations. The GFP PIU and the ratio was calculated and is shown. f The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP⁺ treated with STIF (SCF + TPO + IGF-2 + FGF-1) combinations is shown. The data are a summary from three independent experiments. Error bars represent SEM