Fig. 5

Tet2^−/−;Flt3^ITD hematopoietic precursors underwent primarily symmetric renewal ex vivo. a Representative data from FACS analysis of wild-type (WT) Evi1-GFP and Tet2^−/−;Flt3^ITD;Evi1-GFP HSCs. The cells were stained with antibodies to lineage, Sca1, and c-Kit markers. The lineage negative population was gated first. Numbers indicate percent cells within Lin-c-Kit⁺Sca1⁺ gates. b Representative FACS data from GFP⁺ population from wild-type and Tet2^−/−;Flt3^ITD;Evi1-GFP mouse. The lineage, Sca1, and c-Kit markers were stained and gated first, then the GFP⁺ population from Lin-Sca1⁺c-Kit⁺ was compared with wild-type Evi1-GFP and Tet2^−/−;Flt3^ITD;Evi1-GFP mouse. c The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP⁺ from wild-type Evi1-GFP mouse bone marrow is shown. The data are a summary from three independent experiments. Error bars represent standard error of the mean (SEM). d The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of GFP⁺ cells from Tet2^−/−;Flt3^ITD;Evi1-GFP compound mouse is shown. The data are a summary from three independent experiments. Error bars represent SEM. e The frequency of symmetric self-renewal, symmetric differentiation, and asymmetric division of 500 nM 5-aza-2′-deoxycytidine (DAC) treated GFP⁺ cells from Tet2^−/−;Flt3^ITD;Evi1-GFP compound mouse is shown. The data are a summary from three independent experiments. Error bars represent SEM