Fig. 2

Influence of curcumin on bone marrow-derived mesenchymal stem cell (BMSC) proliferation activity. BMSCs treated with curcumin at a concentration of 0.5 μM (CS-C) and without curcumin (CS-N) for 1 day (a), 3 days (b), 6 days (c), and 9 days (d), respectively. The cell cycle was determined by flow cytometry. e Flow cytometry to assess the cell cycle at the indicated intervals (n = 3). f Quantification of the number of MSCs at the S, G2, and M phase. g Immunofluorescence staining of the proliferation of cells with Ki67 in the CS-N and CS-C groups (cultured for 6 days). h Quantification of the rate of Ki67-positive cells in CS-N and CS-C groups (n = 4). Student’s t test: *P < 0.05, **P < 0.01, ***P < 0.001