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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Evaluation of ex vivo produced endothelial progenitor cells for autologous transplantation in primates

Fig. 2

Phenotypic and functional analysis of EPCs produced from mobilized human PB CD34+ cells. The characterization of the generated EPCs/ECs was analyzed by mature endothelial cell markers CD31 and CD144, NO production measurement, and CD31 and FVIII double staining. a Representative flow cytometry profiles of CD31 and CD144 cell markers on days 0, 12, 21, and 36. b Measurement of nitric oxide (NO) concentration and endothelial NO synthase (eNOS) viability during the culture process. c Immunofluorescence analysis of CD31 on cell surface (red) and FVIII in cytoplasm (green) on day 21 (scale bar = 20 μm)

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