Fig. 5
Autologous transplantation of induced EPCs/ECs into the livers of cynomolgus nonhuman primates. The cultured and labeled nonhuman primate EPCs/ECs were transplanted to livers with sinusoidal endothelium injury at a dose of 3 × 108/500 μL saline in each nonhuman primate through hepatic portal vein injection. The hepatic tissues were sectioned on day 7 (Trans-7d; n = 3) and day 14 (Trans-14d; n = 4) post-transplantation, as well as from the control group (n = 3). a The technical method of autologous transplantation in nonhuman primates. b The GFP- and FITC-microbead-labeled cells were detected in cross-sections of nonhuman primate livers; scale bar indicates 50 μm. c The percentages of transplanted cells were calculated (25 sections per nonhuman primate). The data represent means ± SD; **P < 0.01 compared with the control group. EC endothelial cell, EPC endothelial progenitor cell, FITC fluorescein isothiocyanate, G-CSF granulocyte-colony stimulating factor, GFP green fluorescent protein, PBMC peripheral blood mononuclear cell, PBS phosphate-buffered saline, SCF stem cell factor