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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Delivery of VEGFA in bone marrow stromal cells seeded in copolymer scaffold enhances angiogenesis, but is inadequate for osteogenesis as compared with the dual delivery of VEGFA and BMP2 in a subcutaneous mouse model

Fig. 5

Bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor A (VEGFA) co-expression-mediated bone formation is associated with upregulation of osteogenic and angiogenic markers in the in vivo scaffold explants. Custom PCR array was used to examine differentially expressed osteogenesis- and angiogenesis-related genes in scaffold explants at 2 weeks. ANOVA revealed upregulation of a number of osteogenesis- and angiogenesis-related genes such as a ALPL, b RUNX2, c SPP1 (osteopontin), d ANGPT1, and e PGF in ad-BMP2 + VEGFA explants compared with the ad-GFP and ad-VEGFA explants. ANOVA test with Bonferroni post hoc analysis was undertaken for statistical analysis. **p < 0.01, **p < 0.05. GFP green fluorescent protein, ns not significant

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Stem Cell Research & Therapy

ISSN: 1757-6512