Fig. 5

Comparison of effect of TH and BMP-2 on osteogenic induction of DPSCs. a DPSCs cultured in OM with BMP-2 (300 ng/ml) or TH (10^–6 M). After 7 or 14 days, cells were subjected to ALP staining to detect ALP activity (n = 4). b RT-PCR performed to measure expression levels of osteogenic differentiation markers in DPSCs treated with BMP-2 or TH at day 7 or day 14 (n = 4). Error bars represent SD. Statistical analyses performed by one-way ANOVA (*p < 0.05, **p < 0.01). d day, DPSC human dental pulp stem cell, OM osteogenic medium, TH 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide, BMP bone morphogenic protein