Fig. 6
Radiological findings of mouse calvarial bone defects grafted with TH-induced DPSC sheets at 8 weeks after surgery. a Experimental procedure. DPSC sheets cultured on temperature-responsive dishes in OM with or without TH (10–6 M) for 2 weeks and then transplanted into 3.5-mm defects of mouse calvaria. At 8 weeks after transplantation, calvaria were dissected. b Micro-CT images (top) and color-mapped images created from BMD values (bottom). c Quantification of BV, BMC, and BMD of 5 × 5 × 3-mm3 cuboid samples with a circular defect in the center. Data shown as mean ± SD of five mice per group. *p < 0.05 by Student unpaired two-tailed t test. DPSC human dental pulp stem cell, OM osteogenic medium, TH 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide, BMD bone mineral density, BV bone volume, BMC bone mineral content