Table 1 Primers used for reverse transcription-polymerase chain reaction to establish the undifferentiated state of mouse embryonic stem cell line E14TG2A
From: Embryonic stem cell-derived cardiomyocytes for the treatment of doxorubicin-induced cardiomyopathy
Gene product | Sense primer sequence (orientation: 5′–3′) | Antisense primer sequence (orientation: 3′–5′) | Size (base pairs) |
|---|---|---|---|
Oct3/4 | AGCCTGAGGGCGAAGCAGGA | CCCCAGGGTGAGCCCCACAT | 236 |
Sox2 | AGCTACAGCATGATGCAGGA | GGTCATGGAGTTGTACTGCA | 126 |
Nanog | CAGCCCTGATTCTTCCACCAGTCCC | TGGAAGGTTCCCAGTCGGGTTCACC | 391 |
β-actin | CATCACTATTGGCAACGAGCG | ATGGATGCCACAGGATTCCA | 85 |