Fig. 1
From: A biomaterials approach to influence stem cell fate in injectable cell-based therapies
Effect of ejection rate on osteogenic differentiation capacity of hMSCs ejected via 30G needles, cultured in bipotential osteogenic/adipogenic media for 21 days. a Representative bright-field and fluorescence microscopic images showing ejected hMSCs after culturing in bipotential media. Cell nuclei stained red (PI), mineralised areas stained green (OsteoImage™). Intracellular lipid accumulation indicated by arrows (scale bar = 100 μm). b OsteoImage™ fluorescence values for ejected hMSCs at day 21. Each bar represents mean fluorescence values ± SD (n  =  9 in three independent experiments and two donors). c Number of cells at day 21 quantitated using PI staining of hMSCs from two donors (mean ± SD, n  =  6). d OsteoImage™ fluorescence readings normalised to cell count, based on PI staining (mean ± SD, n  =  6). Statistically significant difference from directly plated control using ANOVA and Dunnet’s post-hoc test: *p ≤ 0.05, ***p ≤ 0.001. PI propidium iodide, RFU relative fluorescence unit, Unind uninduced, Ctrl control