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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: A biomaterials approach to influence stem cell fate in injectable cell-based therapies

Fig. 6

Impact of biomaterial-based carriers on osteogenic differentiation markers of hMSCs ejected via 30G needles, cultured in bipotential media for 21 days. a OsteoImage™ fluorescence values, showing hydroxyapatite formation. Each bar represents mean fluorescence value ± SD, n  =  6 in three donors. Statistically significant difference relative to PBS using ANOVA and Dunnet’s post-hoc test: *p ≤ 0.05, **p ≤ 0.01. b OsteoImage™ fluorescence intensity readings normalised to PI-based cell counts (mean ± SD, n  =  6). c Ejected versus directly plated hMSCs, suspended within collagen and ECM, assessed for osteogenic differentiation capacity. ‘Ejected’ cells were ejected at 10 μl/min, and ‘plated’ cells were 60% of ejected cell number directly plated in 12-well plates. Results are mean fluorescence values ± SD, n  =  4 in two donors. (d) OsteoImage™ fluorescence intensity readings in (c) normalised to PI-based cell counts (mean ± SD, n  =  4 in two donors). e Media alkaline phosphatase (ALP) activity levels of hMSCs ejected at 10 μl/min, via 30G needles, cultured in bipotential osteogenic/adipogenic media for 2 days after adding differentiation media 24 h post ejection. Values shown are mean ± SD (n = 3 in two donors). Statistically significant differences in ALP levels relative to control (Friedman test with Dunn’s post-hoc test): *p < 0.05. f Released ALP levels in ejected versus directly plated hMSCs suspended within collagen and ECM. ‘Ejected’ cells were ejected at 10 μl/min, and ‘plated’ cells were 60% of the initially ejected cell number directly plated in 12-well plates. Results are mean fluorescence values ± SD (n  =  4 in two donors). RFU relative fluorescence unit, Ctrl control, PBS phosphate buffer saline, CMC carboxymethyl cellulose, ECM extracellular matrix, Coll collagen

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