Fig. 1

Cpt1b and palmitoylcarnitine enhance reprogramming efficiency. a Quantitative RT-PCR analysis of cpt1b mRNA levels in mouse embryonic fibroblasts (MEFs) and induced pluripotent stem cells (iPSCs). b Strategy of the PiggyBac Transposon derived reprogramming process and the relative levels of alkaline phosphatase (AP)-positive colonies in the primary reprogramming system with or without cpt1b overexpression. c Strategy of the reprogramming process in the secondary reprogramming system and the relative levels of AP- and green fluorescent protein (GFP)-positive colonies with or without cpt1b overexpression. d Images of AP-stained colonies in control and palmitoylcarnitine (PC)-treated plates. e Relative levels of AP-positive colonies with or without PC (25 μM or 50 μM) in the primary reprogramming system. f Relative levels of AP- and GFP-positive colonies with or without PC (50 μM) in the secondary reprogramming system. Data are presented as the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.005 (Student’s t test). Cpt carnitine palmitoyltransferase, Dox doxycycline, WT wild-type