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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Fatty acid oxidation promotes reprogramming by enhancing oxidative phosphorylation and inhibiting protein kinase C

Fig. 2

Palmitoylcarnitine induced pluripotent stem cells (PC-iPSCs) have the same pluripotency as embryonic stem cells (ESCs) and iPSCs. a Immunofluorescence staining for pluripotent markers in PC-iPSCs and iPSCs (blue is Hoechst for nucleus, red is Nanog, green is Oct4). Scale bar = 100 μm. b Quantitative RT-PCR analysis of pluripotent markers for ESCs (B6/Blu), iPSCs, and PC-iPSCs. c Quantitative RT-PCR analysis of markers for three germ layers in embryonic bodies (EBs) formed by ESCs, iPSCs, and PC-iPSCs. Gata6: endoderm; FGF5, Otx2: ectoderm; Brachyury, Sox4: mesoderm. d Pearson correlation coefficient analysis of global gene expression in RNA-seq analysis performed on mouse embryonic fibroblasts (MEFs), ESCs, iPSCs, and PC-iPSCs. e Heat map of pluripotent gene expression in RNA-seq analysis performed on MEFs, ESCs, iPSCs, and PC-iPSCs (detailed expression data are shown in Additional file 7). Data are presented as the mean ± SEM (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512