Fig. 4

Fatty acid oxidation enhances OXPHOS during early reprogramming. a Relative levels of AP-positive colonies with palmitoylcarnitine (PC) at different stages (1–7 days (7d), 1–14 days (14d), and 1–21 days (21d)) after reprogramming. b Mitostress test of reprogramming mouse embryonic fibroblasts (MEFs) shows the basal oxygen consumption rate (OCR) and the maximal oxidative phosphorylation (OXPHOS) capacity. c Statistical results of maximal OCR levels of (b). d Mitostress test showed the increased maximal OXPHOS capacity after PC treatment (3 days) in normal MEFs and reprogramming MEFs. e Statistical results of maximal OCR levels of (d). f Relative levels of AP-positive colonies with PC in different stages (1–3 days, 1–7 days, and 1–21 days) after reprogramming. Data are presented as the mean ± SEM (n = 3 in a and f, n = 5 in b–e). *P < 0.05 (Student’s t test). Dox doxycycline